Protein Method Blocking Buffers
Alkali Scientific Blocking Agent, Instant nonfat Immunoblotting Grade Dry milk, 32 oz Jar, 1/ea
CellPro™ Blocking Agent, Instant Nonfat Immunoblotting Grade Dry Milk, is a high-quality, ready-to-use solution specifically formulated for use in immunoblotting applications. Designed to deliver exceptional performance and reliability, this blocking agent is an essential component in the preparation and processing of Western blot assays.
Leica Biosystems PEROXIDASE BLOCK NOVOCASTRA
Novocastra Peroxidase Block, RE7101, is intended for use in the peroxidase based immunohistochemical (IHC) staining procedures. The presence of pseudoperoxidase (erythrocytes) and endogenous peroxidase in paraffin sections to be stained by immunoperoxidase procedures, can result in nonspecific staining. A method for the blocking of pseudoperoxidase was described (StreefkerkJG, Journal of Histochemistry and Cytochemistry. 20: 829 (1972)). This product is used in a peroxidase based IHC procedure. Incubating sections with Novocastra Peroxidase Block, RE7101, can neutralize endogenous peroxidase activity. 25 mL of reagent is supplied.
Genetex Inc Trident Universal Protein Blocking Reagent (animal serum free)
The Trident Universal Protein Blocking Reagent (animal serum free) is in a ready to use format. Western blot: Immerse the filter in a sufficient amount of Blocking Reagent and incubate for 30 minutes at RT with gentle shaking. IHC/ICC: Incubate tissue sections/ cell smears in Blocking Reagent for 10 minutes at RT or 37ºC. Note: Optimal incubation times should be determined by the researcher.
LAB BIOREAGENTS StrongBlock III Buffer 250ml
Lab Bioreagents StrongBlocks (I, II, III) are newly developed blocking buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays The blocking buffer is to block excess binding sites on the micro-plate for reducing non-specific binding signal, by which the assay sensitivity and specificity are improved. As many factors can influence nonspecific binding, there is no single blocking buffer that is ideal for each assay. For example, depending on the recognition of detection probes from different assays, one individual blocking buffer may cross-react with detection probe. StrongBlocks (I, II, III) are a variety of buffers designed by different blocker components. During the assay, the test different blocking buffers helps to develop a immunoassay with low background noise and high signal-to-noise.
Cell Signaling Technology Animal-Free Blocking Solution(5X) 250 ml
Animal-Free Blocking Solution(5X) 250 ml
Cell Signaling Technology Phosphate Buffered Saline with Tween 20 (PBST-20X) 1000 ml
Phosphate Buffered Saline with Tween 20 (PBST-20X) 1000 ml
Diagnostic Biosystems BACKGROUND BLOCKER Background Blocker (1X)
To block nonspecific background staining observed during immunostaining.\n\nBackground Blocker is unique serum-free blocking solution used to reduce nonspecific background staining. It is a universal blocking agent which eliminates the need to match species with the link antibody. This blocking reagent has been shown to be more superior than serum-based blocking solutions. In addition to immunihistochemistry, Background Blocker can also be used for ELISA and Western Blot techniques.
Biotium TrueBlack WB Blocking Buffer Kit
The TrueBlack WB blocking buffer is a buffer system designed to achieve optimal specificity and sensitivity by blocking background from the non-specific interaction between the fluorophore-labeled antibodies and the membrane with cell lysates. For blocking and antibody dilution for 50 membranes.
Chondrex Inc ChonBlock Blocking/Sample Dilution ELISA Buffer 100mL
100 mL of a mammalian protein-based ELISA buffer for ELISA plate blocking or ELISA sample dilution that eliminates background noise reactions caused by non-specific reactions of antibodies in serum/plasma samples. Please visit www.chondrex.com or email Chondrex, Inc. at support@chondrex.com for more information on how ChonBlock can improve your ELISA results. pH 7.8. Contains 0.4% Tween-20 and 0.04% ProClin 300.
LICOR Intercept T20 (PBS) Antibody Diluent (500 mL)
Intercept T20 (PBS) Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects.The diluent contains Intercept (PBS) Blocking Buffer preformulated with Tween 20 for use in a phosphate-buffered saline (PBS) system. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (PBS) Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
LICOR Intercept (PBS) Blocking Buffer (10 x 500mL)
Intercept (PBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (PBS) Blocking Buffer is the blocking buffer of choice for near-infrared fluorescent applications when using a phosphate-buffered saline (PBS) system. It contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies, it is an excellent choice for biotin-streptavidin detection and it is suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays. Intercept (PBS) Blocking Buffer is a ready-to-use blocker formulation in PBS. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection. It is stored at 4 °C and is also available in kits with Immobilon-FL PVDF and nitrocellulose membranes.
Cell Signaling Technology Tris Buffered Saline with Tween 20 (TBST-10X) 1000 ml
Tris Buffered Saline with Tween 20 (TBST-10X) 1000 ml
Biogenex Laboratories Power Block
Power Block ™ reagent is a highly effective blocking reagent capable of reducing non-specific background staining associated with immunodetection techniques such as immunocytochemistry, ELISA, and Western blotting. Antibodies tend to bind non-specifically to membranes and tissues. To minimize this nonspecific binding, a blocking buffer is used to enhance signal-to noise ratio. Power Block™ reagent shows no cross-reactivity and it blocks more effectively than serum albumins. The blocking potency of Power Block™ reagent is superior to most other conventional formulations in producing a high signal-to-noise ratio. Power Block™ reagent may also be used as effective diluents for antibodies.""""