Protein Method Blocking Buffers
Cell Biolabs Inc PhosphoBLOCKER™ Blocking Reagent, 200 g dry blend (4 Liters prepared)
The utilization of anti-phosphoprotein antibodies in western blotting has become a commonly used tool for signal transduction research. Unfortunately, low levels of endogenous phosphoprotein in various cell lysates often can not be detected, even with high concentrations of antibody and long exposure times. Most commercially available western blot blockers (e.g. dry milk, serum) are sufficient to block the unreacted sites on the membrane, reducing the amount of nonspecific antibody binding during the assay; however, they are not designed to preserve phosphoprotein antigens during blotting. Cell Biolabs’ PhosphoBLOCKER™ contains a proprietary formulation that provides several advantages over conventional blockers: (1) It is designed specifically for phosphoprotein blotting; (2) It enhances low level phosphoprotein signal without increasing background; and (3) it is an easy to use premixed dry blend. 200 grams (reconstituted to 4 liters).
Molecular Devices Blocking Buffer, 5X Concentrate, 120 mL, Liquid
Concentrated 5X blocking buffer formulated for fluorescence western blot detection. Dilute to 1X for membrane blocking prior to primary antibody incubation to reduce background and improve signal-to-noise in fluorescence-based assays.
- Concentrated 5X formulation.
- Dilutes to 1X for use as a blocking solution.
- Supplied as a 120 mL bottle.
- Reduces background and improves signal-to-noise in fluorescence western blots.
- Compatible with membrane blocking prior to antibody incubations.
- For research use only; not for clinical or diagnostic use.
Bioworld Denhardt's Solution (100X), 50 mL
Denhardt's Solution, 100X Concentrate Acts as a blocking reagent in hybridizations to help prevent probe binding to open membrane space and non-target nucleic acids. Specifications:Ultra Pure GradePurity: 99.0%pH @ 25°C: 5.0-7.0 Conductivity @ 25°C (µmhos): <350 Abs @ 278nm (5% water): 0.600-0.750 DNAse: None detectedRNAse: None detectedStorage: -15 to -22°C
Carbosynth LLC. HAMA blocking reagent, 1 gram
HAMA Blocking Reagent for use in immunoassays such as ELISA
Biotang Inc BLOTTO/TWEEN 20 IN TBS 250 ML, IBB-161. 5% nonfat dry milk, 0.05% Tween-20 in TBS (50 mM Tris-HCl and 150 mM NaCl, pH 7.4 +/- 0.2). Prepared in 18.2 megohms water and filtered through 1 micron filter.
BLOTTO/TWEEN 20 IN TBS 250 ML, IBB-161. 5% nonfat dry milk, 0.05% Tween-20 in TBS (50 mM Tris-HCl and 150 mM NaCl, pH 7.4 +/- 0.2). Prepared in 18.2 megohms water and filtered through 1 micron filter.
Bioworld Blotto in TBS, 1 L
Blotto in TBS Blot blocking buffer used to block non-specific binding for antibodies on the membrane Technical Information: 5% nonfat dry milk, 0.02% Sodium azide in TBS
Sigma Aldrich Fine Chemicals Biosciences Phosphate Buffered Saline with 0.05% TWEEN 20, pH 7.4, 10 Pack
Phosphate Buffered Saline with 0.05% TWEEN 20, pH 7.4, 10 Pack
Genetex Inc Trident Universal Protein Blocking Reagent (animal serum free)
The Trident Universal Protein Blocking Reagent (animal serum free) is in a ready to use format. Western blot: Immerse the filter in a sufficient amount of Blocking Reagent and incubate for 30 minutes at RT with gentle shaking. IHC/ICC: Incubate tissue sections/ cell smears in Blocking Reagent for 10 minutes at RT or 37ºC. Note: Optimal incubation times should be determined by the researcher.
Research Products International Corp BSA Block Buf Sol wAzide 200ml
BSA Blocking Buffer Solution in TBS with Azide, 200 Milliliters
Seracare Life Sciences Inc 10X DULBECCO S PBS W/ TWEEN 20
10X Phosphate-buffered Saline (PBS) is ideal for use in sample preparation and as a wash buffer in general immunoassay applications as well as tissue and cell culture procedures. This modified Dulbecco's buffer is not formulated with magnesium or calcium salts. Tween 20, a non-ionic detergent, reduces non-specific binding and protein-protein interaction during the wash step in immunoassay procedures. pH=7.4. Conductivity controlled.
Surmodics Inc StabilBlock Immunoassay Stabilizer 50ml
StabilBlock™ Immunoassay Stabilizer effectively preserves the conformation and activity of dried proteins while simultaneously blocking the surface to reduce non-specific binding. It can be used to stabilize antibodies, antigens or enzymes on an assortment of immunoassay components including polystyrene plates, tubes, glass, membranes, and filter paper. The key benefits are reduction of non-specific binding while increasing signal-to-noise ratios within immunoassay applications.
Cell Signaling Technology Animal-Free Blocking Solution(5X) 250 ml
Animal-Free Blocking Solution(5X) 250 ml
Amsbio LLC ChonBlock™ Blocking/Sample Dilution ELISA Buffer- 100 ml
ChonBlock Blocking/Sample Dilution ELISA Buffer, 100 ml