Protein Method Blocking Buffers
Bioworld NFDM Diluent/Blocking, 1 L
NFDM Diluent/Blocking 4X, in a priority physiological saline borate buffer is used to minimize non-specific binding of antibody Technical Information:pH 7.6Sold as 10 x 100mL.
As One International Inc 50x Glass Blocking agent, 3 x 1.25 ml
One extra challenge using the LightCycler instrument is that the PCR reagents can form precipitates on the glass capillary surface as the Real time PCR progresses. To prevent this event AS ONE offers a special reagent for blocking the glass capillaries during the Quantitative PCR reaction. The Glass blocking agent is a 50x solution (1 ul per 50 ul PCR reaction).
Bioworld BSA-BLOCKING BUFFER IN TBS
This item has a minimum order quantity of 2 per supplier requirements. BSA-Blocking Buffer in TBS (10X) Blot blocking buffer used to block non-specific binding site for antibodies on the membrane 1x Solution Contains: 3% BSA in TBS (pH 7.4); Sodium Azide (0.02%).
Medchemexpress LLC KRCA-0008 | 1472795-20-2 | 96.2% | 609.12 | 5 MG
KRCA-0008 is a selective ALK/Ack1 inhibitor that can be used for cancer research. It demonstrates high potency against ALK (wt), ALK L1196 M, ALK C1156Y, ALK F1174L, ALK R1275Q, and insulin receptor. This compound is known to inhibit ALK-dependent signaling pathways more potently than crizotinib, induce cell apoptosis, and affect the cell cycle.
- Selective ALK/Ack1 inhibitor with IC50 values of 12 nM for ALK and 4 nM for Ack1.
- Potent against various ALK mutations and insulin receptor.
- Inhibits ALK-dependent signaling pathways.
- Induces cell apoptosis and affects the cell cycle.
- Suppresses tumor growth in an ALK-positive Karpas-299 xenograft model in vivo.
- Useful for cancer research applications.
Bioworld BBS-Casein (10X), 200 mL
BSS-Casein, supplied in 10X PBS and 5% Csein. Technical Information:10X Concentrate has a working pH of 8.5 ( +/- 0.1)Formulated in a borate buffered physiologic saline bufferSold as 2 x 100mL or 500mL.
Boston Bioproducts Inc Tris Buffered Saline-Tween, TBST (10X, with 1% Tween-20, pH 7.4) - 1L
10X Tris Buffered Saline - Tween (TBST) is a buffer-surfactant solution supporting a stable pH range between 7.4 and 8.0. When combined in a variable ratio, TBST is an effective wash buffer in Western blotting, in situ hybridization procedures, and immunohistochemistry staining, as it minimizes non specific binding of the materials without disrupting antigen-antibody binding. TBST is also used as an antibody diluent in immunoassays, and supports an array of molecular biology techniques.
Biocare Medical, LLC Nuclear Decloaker, 10X
Nuclear Decloaker is a Tris buffer heat retrieval solution, pH 9.5. It is specially designed for nuclear antigens including ER, PR, Ki-67, p53, cyclin D1, TdT and TTF-1. Other antibodies that show improved staining include ALK1, ALKc, bcl-2, CA125, CD3 (M), CD15, CD30, CD31, CD79a, MART-1, Tyrosinase and Villin. Nuclear Decloaker can be used with Biocare's digital electric pressure cooker (Decloaking Chamber), a steamer, a water bath, or a microwave oven. Nuclear Decloaker incorporates Assure technology, a color-coded high temperature pH indicator solution. The end-user is assured by visual inspection that the solution is at the correct dilution and pH. This product is specially formulated for superior pH stability at high temperatures and will help prevent the possibility of losing pH sensitive antigens. Nuclear Decloaker is non-toxic, non-flammable, odorless and sodium azide and thimerosal free.
Seracare Life Sciences Inc 10X PBS W/ TWEEN 20(PBST)200ML
10X Phosphate-buffered Saline with Tween 20 (PBST) is ideal for use as a wash buffer in general immunoassay procedures. Tween 20, a non-ionic detergent, reduces non-specific binding and protein-protein interaction during the wash step in immunoassay procedures. pH=7.4. Conductivity controlled.
Bioworld Blotto/Tween in PBS, 250 mL
Blotto/Antifoam in PBS Blot blocking buffer used to block non-specific binding site for antibodies on the membrane Technical Information: 5% nonfat dry milk, 0.05% Tween, 0.02% Sodium azide in PBS
Cell Signaling Technology Phosphate Buffered Saline with Tween 20 (PBST-20X) 1000 ml
Phosphate Buffered Saline with Tween 20 (PBST-20X) 1000 ml
Biotium Permeabilization and Blocking Buffer (5X)
Concentrated buffer stock solution (5X) for one step permeabilization and blocking for immunofluorescence staining. Note: this buffer contains goat serum and therefore is not compatible with anti-goat and anti-sheep secondary antibodies. We recommend Permeabilization Buffer for use with anti-goat and anti-sheep secondary antibodies.
Meso Scale Discovery MSD Blocker D-R, 1 mL, for Research Use Only
The MESO SCALE DISCOVERY Blocker D-R is a specialized blocking reagent designed for research applications. It is essential for enhancing specificity and minimizing non-specific binding in various immunoassays, which in turn improves assay performance and data reliability. This product is provided in a 1 mL volume and is strictly for research use only, not intended for diagnostic procedures.
- Specialized blocker for immunoassays
- Optimizes assay performance
- Reduces non-specific binding
- Available in a 1 mL volume
- Research use only
- Not intended for diagnostic use
- Storage condition of ≤-10 °C
- Made-to-order availability
- Usually ships in 1-3 days
Carbosynth LLC. HAMA blocking reagent, 1 gram
HAMA Blocking Reagent for use in immunoassays such as ELISA
Biotium Napsin-A(NAPSA/1238), CF740 conjugate, 0.1mg/mL
Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B. There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
LICOR Intercept T20 (PBS) Antibody Diluent (10 x 500 mL)
Intercept T20 (PBS) Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects.The diluent contains Intercept (PBS) Blocking Buffer preformulated with Tween 20 for use in a phosphate-buffered saline (PBS) system. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (PBS) Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.